Magdana Surguladze
Agricultural University of Georgia, Georgia
Title: Phytoalexins Stilbenoids in leaves of V. vinifera L. cv. Cabernet Sauvignon as affected by downy mildew (Plasmopara viticola Berl.) infection
Biography
Biography: Magdana Surguladze
Abstract
Stilbenoids are characterized by various and high biological activity. From there activity spectrum for plants is important phytoalexin nature, which occurs in condition of fungicidal and bacterial infections. Vine stilbenoids as phytoalexins and changing there physiological concentrations in condition of bacterial (Agrobacterium tumefaciens) and fungicidal (Plasmopara viticola, Botrytis cinerea, Uncinula necator) diseases are studied by us in Georgian red and white grape variety. It was established stress-metabolite stilbenoids [1—5]. One of them is presented below in research. Changes of leaf stilbenoid concentration of the red grape variety Cabernet Sauvignon under downy mildew infection were studied. Samples of healthy and infected leaves were taken from a 35- year-old vineyard, located in the Kakheti region (East Georgia), during July 2019. The total fraction of stilbenoids was isolated by extraction with ethyl acetate, a “ Sephadex G25” column was utilized and the analysis was done by HPLC/MS method. Downy mildew infection affected the stilbenoid leaf concentration as compared to healthy leaves. trans-Resveratrol, trans-ε-viniferin and cis-piceid were the major detected stilbenoids. trans-Resveratrol was higher in infected leaves while trans- ε-viniferin and cis- piceid were lower, as compared to healthy leaves.