Doriana (Bode) Xhulaj is a researcher at Institute of Plant Genetic Resources, part of Agricultural University of Tirana, Albania. She has a doctorate degree in Plant Biology Conservation from the Faculty of Natural Science of Tirana, and is author of several papers in this field. Currently is involved in different project on plant breeding program.
The experiment was carried in the laboratory of plant tissue cultures, of the Institute on Plant Genetic Resources, Agriculture University of Tirana during 2016. An efficient method was developed for multiple shoot regeneration of wheat from mature embryos without callus formation, for a short period of time (2.5 months). Direct shoot regeneration is important since fewer somaclonal variations are likely to arise in indirect regeneration method. The effect of explants, cutted shoot embryonic meristem and shoot embryonic meristem with scutellum and various combinations of plant growth regulators in MS media on direct shoot regeneration of wheat was investigated. Among different combination of 2,4-D, IBA, NAA and BAP tested, embryo explants cultured in MS medium supplemented with 2 mg/L BAP, 0.6 mg/L 2,4- D resulted in the most efficient direct shoot regeneration and produces a max of 2.5 shoot per explants and 3.4 cm shoot length. Plantlets were successfully transferred to rooting medium. The greatest mean numbers of roots were obtained on MS media supplemented with 0.5 mg/L NAA, and the best mean value of root length (8.2cm) at 0.5 mg/L IBA. In this paper by simple manipulating the concentrations of BAP, 2,4-D, IBA and NAA in the culture medium, is described a method rapidly obtaining whole plants without the subculture of wheat using embryo explants.
Jeom Hwa Han is conducting research on the fruit physiology and ecology to cope with climate change at the Rural Development Administration of Korea. She developed a model for prediction of full bloom date of ‘Niitaka’ pear based on air temperature (2008) and predict full bloom date using this model every year in Korea. Recently, she has a great interest in developing models for estimation of dormancy breaking and yields of deciduous fruit trees.
The grape veraison is a period of rapid change of internal biomaterial, which is closely related to fruit quality change. Therfore, management of grape tree before and after the veraison period is very import for improving the fruit quality, so it is necessary to predict the veraison. We investigated the veraison of ‘Kyoho’ grape cultivated at a temperature gradient of 2.5~6.0℃ higher than the atmospheric condition and developed an prediction model of veraison according to temperature after full bloom. The veraison of ‘Kyoho’ grape was accelerated with higher temperature during the whole growing period, and it was accelerated about 5.1 days when temperature rose by 1℃ . However, after the bloom, the veraison accelerated by about 2.2 days per 1℃ increase. As a result of integrating the temperature from full bloom to veraison in various temperature factors, the coefficient of variation of the daily maximum temperature was the lowest and the mean of integrated daily maximum temperature was 1,681.3℃. Root mean square error(RMSE) of the veraison predicted by applying the integrated daily maximum temperature of 1,681.3℃ was 1.3. I think that it is possible to predict the veraison of ‘Kyoho’ grape by using the predicted air temperature after full bloom.