Salah Abdel-Rahman
Genetic Engineering and Biotechnology Research Institute, Egypt
Title: Detection of adulteration and identification of meat and milk species using molecular genetic techniques
Biography
Biography: Salah Abdel-Rahman
Abstract
PCR and PCR-RFLP techniques were used for rapid and sensitive identification and authentication of species-specific meat (buffalo, camel, cat, cattle, dog, donkey, goat, horse, pig and sheep) and milk products (buffalo, camel, cattle, goat and sheep). PCR products of the gene encoding SSR region were 603, 603, ≤100 and 374 bp in buffalo, cattle, pig and sheep, respectively. For discrimination between buffalo and cattle, the amplified cytochrome b gene (359 bp) was digested by TaqI restriction enzyme. Two fragments 191 and 168 bp were generated in buffalo, whereas no digestion occurred in cattle (359 bp). PCR products were 672, 808, 221 and 221 bp in cat, dog, donkey and horse, respectively. To discriminate between donkey and horse, PCR product of mt-DNA in both donkey and horse (359 bp) was digested by AluI restriction enzyme. Three fragments 189, 96 and 74 bp were generated in horse, whereas no fragments were obtained in donkey (359 bp). PCR amplified fragment size was 300 bp in camel, while the fragment size in goat was 855 bp. The proposed PCR assay represents a quick and sensitive method applicable to the detection and authentication of meat and milk species-specific.